Single-molecule Force Studies

Wesley P. Wong, P.I.

 

publications  

K. Halvorsen, D. Schaak, and W.P. Wong^, (2011), Nanoengineering a single-molecule mechanical switch using DNA self-assembly, Nanotechnology Dec 9;22(49):494005 (2011).


  1. K.Halvorsen, W.P. Wong^, “Massively parallel single-molecule manipulation using

centrifugal force,” Biophysical Journal – Letters 98 (11), L53-L55 (2010). [pdf]


D. Cheng, K. Halvorsen, W.P. Wong^, “High-precision microsphere sorting using velocity sedimentation,” Reviews of Scientific Instruments 81, 026106 (2010). [pdf]


X. Zhang*, K. Halvorsen*, C.-Z. Zhang, W.P. Wong^, and T.A. Springer^, “Mechanoenzymatic cleavage of the ultralarge vascular protein, von Willebrand Factor,” Science 324 (5932), 1330-1334 (2009). [pdf]


E. Evans, K. Halvorsen, K. Kinoshita, and W.P. Wong, “A new approach to analysis of single molecule force measurements,” in Handbook of Single Molecule Biophysics, P. Hinterdorfer, ed., Springer (2009). [link]


W.P. Wong*^ and K. Halvorsen*, "Beyond the frame rate: Measuring high-frequency fluctuations with light intensity modulation," Optics Letters 34 (3), 277-279 (2009). [pdf]


V. Heinrich*^, W.P. Wong*, K. Halvorsen, and E. Evans, “Imaging biomolecular interactions by fast three-dimensional tracking of laser-confined carrier particles,” Langmuir 24, 1194-1203 (2008). [pdf]

K. Halvorsen, “Probing weak single-molecule interactions: development and demonstration of a new instrument,” Ph. D Thesis (advisor: E. Evans), Department of Biomedical Engineering, Boston University, USA (2007). [pdf]

W.P. Wong*^ and K. Halvorsen*, “The effect of integration time on fluctuation measurements: calibrating an optical trap in the presence of motion blur,” Optics Express 14 (25), 12517-12531 (2006). [pdf]

W.P. Wong, “Exploring single-molecule interactions through 3D optical trapping and tracking: from thermal noise to protein refolding,” Ph. D Thesis (advisors: D.R. Nelson and E. Evans), Department of Physics, Harvard University, USA (2006). [pdf]

W.P. Wong^ and E. Evans^, “Rare returns on lost effort,” Nature 437, 198-199 (2005).[pdf]

W.P. Wong, V. Heinrich, and E. Evans, “Exploring reaction pathways of single-molecule interactions through the manipulation and tracking of a potential-confined microsphere in three dimensions,” Mat. Res. Soc. Symp. Proc. 790, P5.1.1-P5.1.12 (2004). [pdf]

W.P. Wong, “Modelling a Tethered Polymer Loop: Shape and thickness compressibility,” Master’s Thesis, The University of British Columbia, Canada (2001). [pdf]


(*contributed equally  ^corresponding author)

SELECTED presentations

W.P. Wong and K. Halvorsen, “Nucleic acid-based linkers for detecting and measuring interactions,”  US provisional patent application 61/556,194 (2011).


W.P. Wong and K. Halvorsen. "(Miniature) Spinning force apparatus,” US

provisional patent application 61350088 (2010).


W.P. Wong and K. Halvorsen, "Spinning force apparatus,” US provisional patent application 12/326,279 (2008).


W.P. Wong and K. Halvorsen, "Methods, apparatus and systems for determining power spectral density of a signal via modulation of the signal,” US provisional patent application 61086,336 (2008).

patents

W.P. Wong, “Single-molecule kinetics under force: probing protein folding and enzymatic activity with optical tweezers,” Rockefeller University (2009).


W.P. Wong, “High-resolution, high-stability, high-frequecy optical tweezers methods with a simple video camera,” Frontiers in Optics, San Jose (2009).


W.P. Wong, “Probing single-molecule kinetics with optical tweezers techniques,” MRSEC Seminar, Brandeis University (2009).


W.P. Wong and K. Halvorsen, “ Probing protein folding kinetics with high-resolution, stabilized optical tweezers,” Biophysical Society Meeting, Boston, MA (2009).


K. Halvorsen and W.P. Wong, “Beyond the frame rate: measuring fast dynamics with a slow camera,” Biophysical Society Meeting, Boston, MA (2009). [pdf]


X. Zhang, K. Halvorsen, W.P. Wong, T.A. Springer, “Unfolding, refolding and proteolysis of the von Willebrand Factor A2 domain under tensile force,” Biophysical Society Meeting, Boston, MA (2009).


W.P. Wong and K. Halvorsen, "Beyond the frame rate: Measuring high-frequency fluctuations with light intensity modulation," New England Complex Fluids Meeting, Harvard University (2008).


W.P. Wong and K. Halvorsen, “Probing protein folding kinetics with optical tweezers,” Gordon Research Conference on Single-molecule Approaches to Biology, Colby-Sawyer College (2008).


W.P. Wong, “Probing protein folding kinetics with single-molecule optical tweezers

techniques,” Linz Winter Workshop, Linz, Austria (2008).


W.P. Wong, “Probing protein folding kinetics with optical tweezers techniques,”  Single-molecule Discussion Club, Harvard University (2008).


W.P. Wong, “Probing single-molecule dynamics and structure with optical tweezers,”   Squishy Physics, Harvard University (2007).


W.P. Wong, K. Halvorsen, K. Kinoshita, and E. Evans, “Using forward and reverse dynamic force spectroscopy to probe unfolding and refolding of poly-spectrin repeats,” Biophysical Society Meeting, Baltimore, MD (2007).